Oxidative Medicine and Cellular Longevity / 2016 / Article / Fig 3

Research Article

Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer

Figure 3

LXA4 negatively regulated cell invasion by inhibiting ROS/ERK pathway. (a) Influence of LXA4 on cell invasion in Panc-1 cells. Cells were treated with vehicle (methanol), LXA4 (400 nM), ROS scavenger NAC (20 mM), or ERK specific inhibitor FR180204 (10 μM) for 24 hours. Then 1 × 105 cells were transferred into transwell chambers covered with Matrigel. After forty-eight hours, cells were stained with 0.1% crystal violet, observed, and counted under microscope. (b) The quantified results of (a). (c) Representative western blot analysis of activated p-ERK and total ERK in cells treated as in (a). (d) Intracellular ROS determined in cells treated in (a). Cells incubated with DCF-DA for 20 min were washed with PBS three times and then lysed by RIPA lysis buffer and tested by fluorimetry at 510 nm. It was normalized by total protein. versus vehicle control.
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