The Antiapoptosis Effect of Glycyrrhizate on HepG2 Cells Induced by Hydrogen Peroxide

Figure 3

Hoechst 33258 staining indicated that GAS inhibits H₂O₂-induced HepG2 cells apoptosis. Morphologic changes in nuclei observed with Hoechst 33258 staining under fluorescence microscopy. (a) Natural group. The HepG2 cells showed normal shape with round intact nuclei; (b) H₂O₂ group, HepG2 cells treated with 1600 μM H₂O₂ for 4 hours, and the obvious morphologic changes were observed; (c) H₂O₂ + Vitamin C (200 nM) group; (d) H₂O₂ + GAS (125 μg/mL) group; (e) H₂O₂ + GAS (25 μg/mL) group; (f) H₂O₂ + GAS (5 μg/mL) group. The arrows indicate apoptotic cells. Original magnification is 400x.