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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 7053451, 15 pages
Research Article

Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells

1Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng, China
2School of Pharmacy, Henan University, Kaifeng, China

Received 18 August 2016; Revised 25 October 2016; Accepted 6 November 2016

Academic Editor: Jacek Zielonka

Copyright © 2016 Juan Cen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Flow cytometry results showed that MCF-7/ADM cells from different sources have higher iROS levels than MCF-7 cells. The ratio was 1.59±0.09 folds (supplementary Figure 1 A) for cells from China Pharmaceutical University (Nanjing, China) and 2.77±0.21 (supplementary Figure 1 B) for cells from Keygen Biotech (Nanjing, China). Moreover, our confocal microscopy results further demonstrated that there was an increase in the fluorescence intensity of DCF, which was evenly distributed all over the MCF-7/ADM cells (supplementary Figure 1 C).

  1. Supplementary Material