HDAC6 Regulates the Chaperone-Mediated Autophagy to Prevent Oxidative Damage in Injured Neurons after Experimental Spinal Cord Injury

Figure 6

HDAC6 affects the activity of CMA by regulating the level of acetylated HSP90. (a) The cell lysates isolated from PC12 and PC12/siHDAC6 cells exposed to HI for 24 h. Ctrl express vehicle-treated group. The protein complexes were immunoprecipitated with anti-acetylation antibody and blotted with anti-HSP90 antibody for determination of acetylated HSP90 (Ac-Hsp90) level. The level of IgG was used as a loading control. (b) The immunocomplexes were precipitated from PC12 and PC12/siHDAC6 cells with anti-HSP90 antibody and blotted with anti-HSC70 or anti-LAMP-2a antibodies for determination of the interaction of HSP90 with HSC70 or LAMP-2a. The level of IgG was used as a loading control.

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