SIRT1/3 Activation by Resveratrol Attenuates Acute Kidney Injury in a Septic Rat Model

Figure 4

Expression of SOD2 protein, acetylation level, and activity in renal tubular epithelial cells after CLP. (a) Representative western blot of SOD2 proteins (upper panel) and densitometric analyses (lower panel); (b) level of acetylated SOD2 was determined using an acetylated SOD2 (Ac-SOD2) antibody normalized to purified SOD2 protein by immunoprecipitation (IP SOD2); (c) SOD2 activity was determined using a SOD2 Assay kit and normalized to that of the control group. Results are expressed as fold change over control. Values shown are the mean ± SEM. and versus the control group; and versus the vehicle group; versus the RSV group. . RSV, resveratrol; Ac-lys, acetylated lysine; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SOD2, superoxide dismutase 2.

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