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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 7510759, 12 pages
http://dx.doi.org/10.1155/2016/7510759
Research Article

Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress

1Department of Endocrinology, 4th Clinical Military Hospital, Rudolfa Weigla 5, 50-981 Wrocław, Poland
2Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland
3Department of Basic Medical Sciences, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland

Received 26 May 2015; Revised 21 August 2015; Accepted 31 August 2015

Academic Editor: Francesca Danesi

Copyright © 2016 Katarzyna Skorkowska-Telichowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Valko, D. Leibfritz, J. Moncol, M. T. D. Cronin, M. Mazur, and J. Telser, “Free radicals and antioxidants in normal physiological functions and human disease,” International Journal of Biochemistry and Cell Biology, vol. 39, no. 1, pp. 44–84, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. K. R. Martin and J. C. Barrett, “Reactive oxygen species as double-edged swords in cellular processes: low-dose cell signaling versus high-dose toxicity,” Human and Experimental Toxicology, vol. 21, no. 2, pp. 71–75, 2002. View at Publisher · View at Google Scholar · View at Scopus
  3. J.-H. Chen, C. N. Hales, and S. E. Ozanne, “DNA damage, cellular senescence and organismal ageing: causal or correlative?” Nucleic Acids Research, vol. 35, no. 22, pp. 7417–7428, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Ding, C. Li, N. Cheng, X. Cui, X. Xu, and G. Zhou, “Redox regulation in cancer stem cells,” Oxidative Medicine and Cellular Longevity, vol. 2015, Article ID 750798, 11 pages, 2015. View at Publisher · View at Google Scholar
  5. J. Hu, Q. Yu, F. Zhao et al., “Protection of Quercetin against Triptolide-induced apoptosis by suppressing oxidative stress in rat Leydig cells,” Chemico-Biological Interactions, vol. 240, pp. 38–46, 2015. View at Publisher · View at Google Scholar
  6. G. Valacchi, C. Sticozzi, G. Belmonte et al., “Vitamin C compound mixtures prevent ozone-induced oxidative damage in human keratinocytes as initial assessment of pollution protection,” PLoS ONE, vol. 10, no. 8, Article ID e0131097, 2015. View at Publisher · View at Google Scholar
  7. M. Czemplik, A. Boba, K. Kostyn et al., “Flax engineering for biomedical application,” in Biomedical Engineering, Trends, Research and Technologies, M. A. Komorowska and S. Olsztynska-Janus, Eds., chapter 17, InTech, Rijeka, Croatia, 2011. View at Publisher · View at Google Scholar
  8. M. D. Alister and W. D. Neil, Eds., Flax: The Genus Linum, Medicinal and Aromatic Plants—Industrial Profiles, CRC Press, 1st edition, 2003.
  9. M. Styrczewska, A. Kulma, K. Kostyn, K. Hasiewicz-Derkacz, and J. Szopa, “Flax terpenoid pathway as a source of health promoting compounds,” Mini-Reviews in Medicinal Chemistry, vol. 13, no. 3, pp. 353–364, 2013. View at Google Scholar · View at Scopus
  10. M. Fujisawa, M. Watanabe, S.-K. Choi, M. Teramoto, K. Ohyama, and N. Misawa, “Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB,” Journal of Bioscience and Bioengineering, vol. 105, no. 6, pp. 636–641, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Gruszka and J. Kruk, “RP-LC for determination of plastochromanol, tocotrienols and tocopherols in plant oils,” Chromatographia, vol. 66, no. 11-12, pp. 909–913, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. L. Velasco and F. D. Goffman, “Tocopherol, plastochromanol and fatty acid patterns in the genusLinum,” Plant Systematics and Evolution, vol. 221, no. 1-2, pp. 77–88, 2000. View at Publisher · View at Google Scholar · View at Scopus
  13. A.-N. Li, S. Li, Y.-J. Zhang, X.-R. Xu, Y.-M. Chen, and H.-B. Li, “Resources and biological activities of natural polyphenols,” Nutrients, vol. 6, no. 12, pp. 6020–6047, 2014. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Zuk, A. Prescha, M. Stryczewska, and J. Szopa, “Engineering flax plants to increase their antioxidant capacity and improve oil composition and stability,” Journal of Agricultural and Food Chemistry, vol. 60, no. 19, pp. 5003–5012, 2012. View at Publisher · View at Google Scholar · View at Scopus
  15. K. Lorenc-Kukula, R. Amarowicz, J. Oszmianski et al., “Pleiotropic effect of phenolic compounds content increases in transgenic flax plant,” Journal of Agricultural and Food Chemistry, vol. 53, no. 9, pp. 3685–3692, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. K. Lorenc-Kukuła, M. Zuk, A. Kulma et al., “Engineering flax with the GT family 1 Solanum sogarandinum glycosyltransferase SsGT1 confers increased resistance to fusarium infection,” Journal of Agricultural and Food Chemistry, vol. 57, no. 15, pp. 6698–6705, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. K. Hasiewicz-Derkacz, K. Kostyn, A. Kulma, T. Gebarowski, K. Gasiorowski, and J. Szopa, “The properties of emulsion based on the ideal oil obtained from seed of transgenic flax plants overproducing phenylpropanoid compounds,” FEBS Journal, vol. 280, pp. 518–519, 2013. View at Google Scholar
  18. M. Czemplik, A. Kulma, K. Bazela, and J. Szopa, “The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene,” BMC Complementary and Alternative Medicine, vol. 12, article 251, 2012. View at Publisher · View at Google Scholar · View at Scopus
  19. K. Skórkowska-Telichowska, M. Zuk, A. Kulma et al., “New dressing materials derived from transgenic flax products to treat long-standing venous ulcers—a pilot study,” Wound Repair and Regeneration, vol. 18, no. 2, pp. 168–179, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. K. Hasiewicz-Derkacz, A. Kulma, T. Czuj et al., “Natural phenolics greatly increase flax (Linum usitatissimum) oil stability,” BMC Biotechnology, vol. 15, no. 1, article 62, 2015. View at Publisher · View at Google Scholar
  21. M. Lukaszewicz, I. Matysiak-Kata, J. Skala, I. Fecka, W. Cisowski, and J. Szopa, “Antioxidant capacity manipulation in transgenic potato tuber by changes in phenolic compounds content,” Journal of Agricultural and Food Chemistry, vol. 52, no. 6, pp. 1526–1533, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Prescha, A. Świȩdrych, J. Biernat, and J. Szopa, “Increase in lipid content in potato tubers modified by 14-3-3 gene overexpression,” Journal of Agricultural and Food Chemistry, vol. 49, no. 8, pp. 3638–3643, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. A. Prescha, M. Grajzer, M. Dedyk, and H. Grajeta, “The antioxidant activity and oxidative stability of cold-pressed oils,” Journal of the American Oil Chemists' Society, vol. 91, no. 8, pp. 1291–1301, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. V. K. S. Shukla, P. C. Dutta, and W. E. Artz, “Camelina oil and its unusual cholesterol content,” Journal of the American Oil Chemists' Society, vol. 79, no. 10, pp. 965–969, 2002. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Jaromin, R. Zarnowski, and A. Kozubek, “Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect,” Cellular and Molecular Biology Letters, vol. 11, no. 3, pp. 438–448, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Li, Z. Cao, and H. Zhu, “Upregulation of endogenous antioxidants and phase 2 enzymes by the red wine polyphenol, resveratrol in cultured aortic smooth muscle cells leads to cytoprotection against oxidative and electrophilic stress,” Pharmacological Research, vol. 53, no. 1, pp. 6–15, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. V. Vichai and K. Kirtikara, “Sulforhodamine B colorimetric assay for cytotoxicity screening,” Nature Protocols, vol. 1, no. 3, pp. 1112–1116, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. E. Eruslanov and S. Kusmartsev, “Identification of ROS using oxidized DCFDA and flow-cytometry,” Methods in Molecular Biology, vol. 594, pp. 57–72, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. S. Roy, S. Khanna, K. Nallu, T. K. Hunt, and C. K. Sen, “Dermal wound healing is subject to redox control,” Molecular Therapy, vol. 13, no. 1, pp. 211–220, 2006. View at Publisher · View at Google Scholar · View at Scopus
  30. A. E. K. Loo and B. Halliwell, “Effects of hydrogen peroxide in a keratinocyte-fibroblast co-culture model of wound healing,” Biochemical and Biophysical Research Communications, vol. 423, no. 2, pp. 253–258, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. A. R. Collins, “The comet assay for DNA damage and repair: principles, applications, and limitations,” Applied Biochemistry and Biotechnology, vol. 26, no. 3, pp. 249–261, 2004. View at Publisher · View at Google Scholar · View at Scopus
  32. K. Gąsiorowski, B. Brokos, A. Kulma, A. Ogorzałek, and K. Skórkowska, “Impact of four antimutagens on apoptosis in genotoxically damaged lymphocytes in vitro,” Cellular & Molecular Biology Letters, vol. 6, no. 3, pp. 649–675, 2001. View at Google Scholar · View at Scopus
  33. V. Sapountzi and J. Côté, “MYST-family histone acetyltransferases: beyond chromatin,” Cellular and Molecular Life Sciences, vol. 68, no. 7, pp. 1147–1156, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. I. B. Wall, R. Moseley, D. M. Baird et al., “Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers,” Journal of Investigative Dermatology, vol. 128, no. 10, pp. 2526–2540, 2008. View at Publisher · View at Google Scholar · View at Scopus
  35. D. M. Kasote, “Flaxseed phenolics as natural antioxidants,” International Food Research Journal, vol. 20, no. 1, pp. 27–34, 2013. View at Google Scholar · View at Scopus
  36. V. Lattanzio, V. M. T. Lattanzio, and A. Cardinali, “Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects,” in Phytochemistry: Advances in Research, F. Imperato, Ed., pp. 23–67, Research Signpost, 2006. View at Google Scholar
  37. S. M. Mandal, D. Chakraborty, and S. Dey, “Phenolic acids act as signaling molecules in plant-microbe symbioses,” Plant Signaling and Behavior, vol. 5, no. 4, pp. 359–368, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. F. Afaq, D. N. Syed, A. Malik et al., “Delphinidin, an anthocyanidin in pigmented fruits and vegetables, protects human HaCaT keratinocytes and mouse skin against UVB-mediated oxidative stress and apoptosis,” Journal of Investigative Dermatology, vol. 127, no. 1, pp. 222–232, 2007. View at Publisher · View at Google Scholar · View at Scopus
  39. J. Neradil, R. Veselská, and J. Slanina, “UVC-protective effect of caffeic acid on normal and transformed human skin cells in vitro,” Folia Biologica, vol. 49, no. 5, pp. 197–202, 2003. View at Google Scholar · View at Scopus
  40. A. L. Ronco and E. De Stéfani, “Squalene: a multi-task link in the crossroads of cancer and aging,” Functional Foods in Health & Disease, vol. 3, no. 12, p. 462, 2013. View at Google Scholar
  41. B. J. Grattan Jr., “Plant sterols as anticancer nutrients: evidence for their role in breast cancer,” Nutrients, vol. 5, no. 2, pp. 359–387, 2013. View at Publisher · View at Google Scholar · View at Scopus
  42. J. K. Winkler and K. Warner, “The effect of phytosterol concentration on oxidative stability and thermal polymerization of heated oils,” European Journal of Lipid Science and Technology, vol. 110, no. 5, pp. 455–464, 2008. View at Publisher · View at Google Scholar · View at Scopus
  43. A. Singh, “Sitosterol as an antioxidant in frying oils,” Food Chemistry, vol. 137, no. 1–4, pp. 62–67, 2013. View at Publisher · View at Google Scholar · View at Scopus
  44. D. J. Ruthig and K. A. Meckling-Gill, “Both (n-3) and (n-6) fatty acids stimulate wound healing in the rat intestinal epithelial cell line, IEC-6,” The Journal of Nutrition, vol. 129, no. 10, pp. 1791–1798, 1999. View at Google Scholar · View at Scopus
  45. B. Lipinski, “Hydroxyl radical and its scavengers in health and disease,” Oxidative Medicine and Cellular Longevity, vol. 2011, Article ID 809696, 9 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  46. M. Darvin, L. Zastrow, W. Sterry, and J. Lademann, “Effect of supplemented and topically applied antioxidant substances on human tissue,” Skin Pharmacology and Physiology, vol. 19, no. 5, pp. 238–247, 2006. View at Publisher · View at Google Scholar · View at Scopus
  47. J. D. Lambert, J. Hong, G. Y. Yang, J. Liao, and C. S. Yang, “Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations,” The American Journal of Clinical Nutrition, vol. 81, no. 1, supplement, pp. 284S–291S, 2005. View at Google Scholar
  48. T. M. Lima, C. C. Kanunfre, C. Pompéia, R. Verlengia, and R. Curi, “Ranking the toxicity of fatty acids on Jurkat and Raji cells by flow cytometric analysis,” Toxicology in Vitro, vol. 16, no. 6, pp. 741–747, 2002. View at Publisher · View at Google Scholar · View at Scopus
  49. A. Vecchini, V. Ceccarelli, F. Susta et al., “Dietary alpha-linolenic acid reduces COX-2 expression and induces apoptosis of hepatoma cells,” Journal of Lipid Research, vol. 45, no. 2, pp. 308–316, 2004. View at Publisher · View at Google Scholar · View at Scopus
  50. S. Serini, E. Piccioni, N. Merendino, and G. Calviello, “Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer,” Apoptosis, vol. 14, no. 2, pp. 135–152, 2009. View at Publisher · View at Google Scholar · View at Scopus
  51. Novartis, Ed., MF59 Adjuvant Fact Sheet, Novartis, 2009.
  52. F. Mosca, E. Tritto, A. Muzzi et al., “Molecular and cellular signatures of human vaccine adjuvants,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 30, pp. 10501–10506, 2008. View at Publisher · View at Google Scholar · View at Scopus
  53. L. Kalvodova, “Squalene-based oil-in-water emulsion adjuvants perturb metabolism of neutral lipids and enhance lipid droplet formation,” Biochemical and Biophysical Research Communications, vol. 393, no. 3, pp. 350–355, 2010. View at Publisher · View at Google Scholar · View at Scopus
  54. A. Azzi, K. J. A. Davies, and F. Kelly, “Free radical biology—terminology and critical thinking,” FEBS Letters, vol. 558, no. 1–3, pp. 3–6, 2004. View at Publisher · View at Google Scholar · View at Scopus
  55. A. Scalbert, I. T. Johnson, and M. Saltmarsh, “Polyphenols: antioxidants and beyond,” The American Journal of Clinical Nutrition, vol. 81, no. 1, supplement, pp. 215S–217S, 2005. View at Google Scholar · View at Scopus
  56. H. Sies, “Polyphenols and health: update and perspectives,” Archives of Biochemistry and Biophysics, vol. 501, no. 1, pp. 2–5, 2010. View at Publisher · View at Google Scholar · View at Scopus