Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress

Table 4

Multicriterial analysis of tested emulsions in the aspect of their enhancement of fibroblasts activity in the culture. The results of six in vitro tests with emulsions added to the final concentration of 0.5% (v/v) of cell culture medium were analyzed. Cultures of V79 fibroblasts were carried out in the presence of tested emulsions for 48 h (test 1). For evaluation of antioxidative activity of the emulsions at the end of culture time cells were exposed to oxidative stress with H₂O₂ [100 M, 30 min, 4°C] (tests: 2–6). The multicriterial analysis was performed according to the method described in Section 2.

	Rating and ranking criteria			Tested emulsions
	Test	Expected result	Index of importance	Linola	W92	W86	GT
(1)	Proliferation of V79 fibroblasts	Increase	0.2	0.123	5.714	13.604	1.235
(2)	Intracellular contents of ROS	Decrease	0.2	1.904	18.182	12.820	6.896
(3)	DNA contents in comets’ head	Increase	0.2	0.188	0.144	0.766	0.268
(4)	Olive tail moment	Decrease	0.2	0.144	0.093	0.130	0.214
(5)	Frequency of apoptosis	Decrease	0.1	0.452	0.038	0.1618	0.022
(6)	Frequency of necrosis	Decrease	0.1	0.011	0.078	0.526	0.171
Total (sum of the results in columns)			1.0	2.8225	24.2495	28.0078	8.8058