Sociodemographic and Lifestyle Determinants of Plasma Oxidative Stress Markers 8-OHdG and F2-Isoprostanes and Associations with Metabolic Syndrome
Table 2
Associations of sociodemographic, lifestyle, and sampling factors with plasma 8-OHdG and F2-isoprostanes.
8-OHdG
F2-isoprostanes
Univariate
Multivariable
Univariate
Multivariable
Sociodemographics
Age (years)
0.16
<0.001
0.16
<0.001
0.07
0.018
0.07
0.043
Female sex (male is ref.)
−0.16
<0.001
−0.14
<0.001
0.13
<0.001
0.15
<0.001
Education (years)
−0.04
0.154
−0.02
0.558
−0.09
0.002
−0.10
0.001
North-European ancestry (“NO” is ref.)
0.05
0.128
0.05
0.129
0.00
0.915
−0.01
0.738
Lifestyle
Smoking
Ref. nonsmokers
Ref.
Ref.
Ref.
Ref.
<10 cig/day
0.08
0.010
0.05
0.120
0.04
0.191
0.02
0.546
≥10 cig/day
0.11
<0.001
0.05
0.398
0.02
0.497
−0.07
0.098
Plasma cotinine (ng/mL)
0.12
<0.001
0.09
0.034
0.07
0.013
0.09
0.027
Alcohol
ref. < 1 unit/wk
Ref.
Ref.
Ref.
Ref.
♂ ≤ 21/♀ ≤ 14 units/wk
0.04
0.233
0.00
0.921
0.01
0.818
0.06
0.094
♂ > 21/♀ > 14 units/wk
0.03
0.326
−0.02
0.553
0.12
<0.001
0.13
<0.001
Body mass index (kg/m2)
0.04
0.160
−0.02
0.594
0.06
0.053
0.05
0.099
Physical activity (MET-min/wk)
0.00
0.925
0.00
0.926
0.00
0.882
−0.02
0.440
Supplement use (“NON user” is ref.)
0.02
0.456
0.01
0.848
−0.05
0.076
−0.07
0.020
Sampling variables
Season of sample collection
Ref. winter (Dec.–Feb.)
Ref.
Ref.
Ref.
Ref.
Spring (Mar.–May)
0.00
0.997
0.02
0.674
0.10
0.005
0.11
0.002
Summer (Jun.–Aug.)
−0.02
0.606
0.01
0.884
0.05
0.153
0.07
0.043
Autumn (Sep.–Nov.)
−0.01
0.877
0.01
0.729
−0.03
0.493
−0.01
0.776
Fasting prior to sample collection (“NO” is ref.)
−0.08
0.010
−0.06
0.034
−0.06
0.050
−0.07
0.014
8-OHdG and F2-isoprostanes were log transformed for analysis. All analyses are adjusted for research site. Results are reported as standardized regression coefficients. Cig: cigarettes; kg: kilogram; m2: meters squared; MET: metabolic equivalent of task; min: minutes; ref.: reference; Wk: week.
