Cell Line-Dependent Variability of Coordinate Expression of p75NTR and CRABP1 and Modulation of Effects of Fenretinide on Neuroblastoma Cells
(a) Western blot for CRABP1 in SH-EP1 cells transfected with an expression construct for p75NTR (OE) or the analogous empty vector (OE Ctrl). Staining for α-tubulin serves as a loading control. The graph below the blot depicts mean optical density and SEM () of each band relative to the optical density of the corresponding band for α-tubulin and normalized to OE Ctrl = 1.00. (b) qRT-PCR for CRABP1 performed on lysates from OE and OE Ctrl SH-EP1 cells ( independent samples each) demonstrates the overexpression of CRABP1 in OE cells relative to OE Ctrl cells (, Student’s -test). (c) Transient knockdown of p75NTR with siRNA in SH-EP1 cells demonstrates coordinate regulation of p75NTR and CRABP1 expression (Western blot; β-actin serves as a loading control). The graph on the right of the blot depicts mean optical density and SEM () of each band relative to the optical density of the corresponding band for β-actin and normalized to control = 1.00.