Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway

Figure 4

SFN modulates expression of inflammatory mediators, NF-κB activity, and NLRP3 inflammasome during cerulein-induced AP. (a) The mRNA levels of pancreatic IL-1β, IL-6, TNF-α, and TGF-β after SFN treatment in cerulein-stimulated pancreatic acinar cells were measured by quantitative real-time PCR. (b, c) Correspondently, the protein levels of IL-1β and TGF-β were determined using quantitative ELISA kits. (d) The effect of SFN on phosphor-p65 protein expression in cerulein-treated pancreatic acinar cells was measured by western blot. (e) NF-κB p65 protein expression in cerulein-induced mice was determined by immunohistochemistry. (f) The effect of SFN on cerulein-induced NLRP3 inflammasome activation was evaluated by detecting the protein levels of NLRP3, caspase-1-p20, and cleaved IL-1β in cerulein-induced acinar cells by western blot assay. Values are expressed as mean ± SEM. and when cerulein-induced group was compared with control group. , , and when SFN-treated group was compared to cerulein-induced group.

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