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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 8194690, 11 pages
http://dx.doi.org/10.1155/2016/8194690
Research Article

Cardioprotective Effects of Astragalin against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart

1Department of Cardiac Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China
2Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Pharmacy School, Shihezi University, Shihezi 832002, China
3Shandong University of Traditional Chinese Medicine, Jinan 250014, China

Received 29 June 2015; Accepted 15 September 2015

Academic Editor: Catherine Brenner

Copyright © 2016 Daoxu Qu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aims to evaluate the cardioprotective effects of astragalin against myocardial ischemia/reperfusion (I/R) injury in isolated rat heart. The cardioprotective effects of astragalin on myocardial I/R injury were investigated on Langendorff apparatus. Adult male Sprague-Dawley rats were randomly divided into five groups. The results showed that astragalin pretreatment improved myocardial function. Compared with I/R group, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in coronary flow decreased in astragalin pretreatment groups, whereas superoxide dismutase (SOD) activity and glutathione/glutathione disulfide (GSH/GSSG) ratio significantly increased. The levels of malondialdehyde (MDA), intracellular reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) decreased in astragalin-treated groups. The infarct size (IS) and apoptosis rate in hearts from astragalin-treated groups were lower than those in hearts from the I/R group. Western blot analysis also revealed that astragalin preconditioning significantly reduced Bax level, whereas Bcl-2 was increased in the myocardium. Therefore, astragalin exhibited cardioprotective effects via its antioxidative, antiapoptotic, and anti-inflammatory activities.