Research Article

Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation

Figure 2

Histogram of green fluorescent intensity for CellROX green and DCFH probes and epifluorescence microscopy of spermatozoa stained with CellROX green. Histogram of green fluorescent intensity for CellROX green (a) and DCFH (b), wherein green lines correspond to control (without H2O2); red = 12,5 μM H2O2; blue = 50 μM H2O2; and orange = 200 μM H2O2. Spermatozoa stained with CellROX green (c), positive (intense green) and negative cells (weak green), 1000x magnification with mineral oil.
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