The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes

Figure 6

(a) Representative examples of Annexin V-FITC Dot-Plots Gating (FL1 LOG versus FITC LOG) of nonirradiated (NI) and UV-B (225 mJ/cm²) irradiated (IR) HaCaT cells not previously exposed or exposed to 10 μM complexed lycopene (Lyc-CD); (b) results are expressed as percentage (mean ± SD, ) of nonapoptotic and viable cells, Q8: Annexin-FTIC (−) and PI (−); early apoptotic cells, Q5: Annexin-FTIC (+) and PI (−); predominantly late apoptotic or dead cells, Q6: Annexin- FTIC (+) and PI (+); and predominantly necrotic and dead cells, Q7: Annexin-FTIC (−) and PI (+). Statistical analysis: One-Way ANOVA with All Pairwise Multiple Comparison Procedures (Holm-Sidak method): means with different letters are significantly different ().

(a)

(b)