Research Article

Hericium erinaceus Inhibits TNF-α-Induced Angiogenesis and ROS Generation through Suppression of MMP-9/NF-κB Signaling and Activation of Nrf2-Mediated Antioxidant Genes in Human EA.hy926 Endothelial Cells

Figure 3

HE suppresses MMP-9 activity and expression in TNF-α-stimulated EA.hy926 cells. Cells were pretreated with 50 and 100 μg/mL HE for 2 h and then stimulated with TNF-α (10 ng/mL) for 24 h. (a) An equal amount (50 μg) of conditioned culture media from each sample was subjected to gelatin zymography. The relative density of MMP-9 bands was measured by commercially available quantitative software. (b) An equal amount (50 μg) of total lysate from each sample was resolved by 10% SDS-PAGE with β-actin as a control. The relative changes in protein bands were measured, the control being 1-fold, as shown just below the gel data. Results are presented as mean ± SD of three independent assays; indicates significant difference compared to control, and indicates significant difference compared to TNF-α alone treatment.