NLRP3 Inflammasome Activation in the Brain after Global Cerebral Ischemia and Regulation by 17β-Estradiol

Figure 8

The ability of E2 to suppress NLRP3-ASC complex formation after GCI is lost in PELP1 forebrain-specific knockout mice. (a) Representative confocal images of Duolink in situ co-IP show that ASC-NLRP3 complex is formed in hippocampal CA1 region at day 6 after GCI. This complex formation is robustly suppressed by E2 in the FLOX but not in KO (KO = PELP1 knockout) (magnification = 40x, scale bar = 50 μm). (b) MATLAB analysis of the images shows a statistically significant suppression of complex formation by E2 in FLOX but not in KO mice (, sham versus GCI; , GCI versus GCI + E2; , FLOX GCI + E2 versus KO GCI + E2; NS, not significant, KO GCI versus KO GCI + E2) ( animals/group).

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