Research Article

The Role of the Exo-Xis Region in Oxidative Stress-Mediated Induction of Shiga Toxin-Converting Prophages

Table 1

Escherichia coli strains.

Strain
Genotype or relevant characteristicsReference

E. coli MG1655   ilvG rfb-50 rph-1[27]
E. coli MG1655 (λ)MG1655 bearing λ prophage [24]
E. coli MG1655 (λΔexo-xis)MG1655 bearing λ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by recombination
E. coli MG1655 (λΔorfs)MG1655 bearing λ prophage with deletion of orf60a, orf63, orf61, and orf73This study, by recombination
E. coli MG1655 (λΔorf60a)MG1655 bearing λ prophage with deletion of orf60a This study, by recombination
E. coli MG1655 (λΔorf63)MG1655 bearing λ prophage with deletion of orf63 This study, by recombination
E. coli MG1655 (λΔorf61)MG1655 bearing λ prophage with deletion of orf61This study, by recombination
E. coli MG1655 (λΔorf73)MG1655 bearing λ prophage with deletion of orf73This study, by recombination
E. coli MG1655 (λΔea22)MG1655 bearing λ prophage with deletion of ea22This study, by recombination
E. coli MG1655 (λΔea8.5)MG1655 bearing λ prophage with deletion of ea8.5This study, by recombination
E. coli MG1655 (Φ)MG1655 bearing Φ prophage[24]
E. coli MG1655 (ΦΔexo-xis)MG1655 bearing Φ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by recombination
E. coli MG1655 (ΦΔorfs)MG1655 bearing Φ prophage with deletion of 4 orfs being homologues of λorf60a, λorf63, λorf61, and λorf73This study, by recombination
E. coli MG1655 (ΦΔorf60a)MG1655 bearing Φ prophage with deletion of vb__9c, the homologue of λorf60a This study, by recombination
E. coli MG1655 (ΦΔorf63)MG1655 bearing Φ prophage with deletion of vb__8c, the homologue of λorf63 This study, by recombination
E. coli MG1655 (ΦΔorf61)MG1655 bearing Φ prophage with deletion of vb__7c, the homologue of λorf61This study, by recombination
E. coli MG1655 (ΦΔorf73)MG1655 bearing Φ prophage with deletion of the sequence of putative C4 zinc finger protein, the homologue of λorf73This study, by recombination
E. coli MG1655 (ΦΔea22)MG1655 bearing Φ prophage with deletion of vb__6c, the analogue of λea22This study, by recombination
E. coli MG1655recA13MG1655 but recA13[33]
E. coli MG1655recA13 (λ)MG1655recA13 bearing λ prophage This study, by lysogenization
E. coli MG1655recA13 (λΔexo-xis)MG1655recA13 bearing λ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by lysogenization
E. coli MG1655recA13 )MG1655recA13 bearing Φ prophageThis study, by lysogenization
E. coli MG1655recA13 Δexo-xis)MG1655recA13 bearing Φ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by lysogenization
E. coli PQ37sfiA::Mud(Ap lac) cts, lacΔU169, mal+, galE, galY, PhoC, rfa, F, thr, leu, his, pyrD, thi, trp::MUC+, srl300::Tn10, rpoB, uvrA+[31]
E. coli PQ37 (λ)PQ37 bearing λ prophage This study, by lysogenization
E. coli PQ37 (λΔexo-xis)PQ37 bearing λ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by lysogenization
E. coli PQ37 (Φ)PQ37 bearing Φ prophageThis study, by lysogenization
E. coli PQ37 (ΦΔexo-xis)PQ37 bearing Φ prophage with deletion of all orfs and genes localized between genes exo and xisThis study, by lysogenization