Research Article

Differential Mitochondrial Adaptation in Primary Vascular Smooth Muscle Cells from a Diabetic Rat Model

Table 1

Mitochondrial respiration and ATP concentrations as measured with O2k from Oroboros and ATP fluorometric assay. Respiration baseline comparisons are between Wistar () and GK () SMCs in NG (5 mM), Wistar, or GK SMCs, passages 8–10. ATP concentrations (µM/1 × 106 cells) are between Wistar () and GK (). Respiration was measured as oxygen flux (pmol second−1 million cells−1) ± standard error of the mean (SEM). as measured by Student’s -test.

Respiration statesWistarGK

S2 (PMG)18.35 ± 3.9729.79 ± 5.38
S3 (PMG)75.99 ± 19.04153.88 ± 23.82
S3 (PMG/S)187.82 ± 40.96241.30 ± 36.46
S4 (PMG/S)54.88 ± 8.3352.52 ± 4.56
Uncoupled262.98 ± 46.06375.15 ± 20.63
RCR3.35 ± 0.294.56 ± 0.45
ATP8.64 ± 1.917.14 ± 1.08