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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 8902954, 9 pages
http://dx.doi.org/10.1155/2016/8902954
Research Article

Effects of Bauhinia forficata Tea on Oxidative Stress and Liver Damage in Diabetic Mice

1Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil
2Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil
3Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, Campus São Gabriel, Avenida Antônio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil
4Departamento de Morfologia, Universidade Federal de Santa Maria, Avenida Roraima 1000, Camobi, 97105-900 Santa Maria, RS, Brazil

Received 25 August 2015; Revised 9 October 2015; Accepted 11 October 2015

Academic Editor: Denis Delic

Copyright © 2016 Andréia Caroline Fernandes Salgueiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was designed to evaluate the effects of Bauhinia forficata Link subsp. pruinosa (BF) tea on oxidative stress and liver damage in streptozotocin (STZ)-induced diabetic mice. Diabetic male mice have remained 30 days without any treatment. BF treatment started on day 31 and continued for 21 days as a drinking-water substitute. We evaluated (1) BF chemical composition; (2) glucose levels; (3) liver/body weight ratio and liver transaminases; (4) reactive oxygen species (ROS), lipid peroxidation, and protein carbonylation in liver; (5) superoxide dismutase (SOD) and catalase (CAT) activities in liver; (6) δ-aminolevulinate dehydratase (δ-ALA-D) and nonprotein thiols (NPSH) in liver; (7) Nrf2, NQO-1, and HSP70 levels in liver and pancreas. Phytochemical analyses identified four phenols compounds. Diabetic mice present high levels of NQO-1 in pancreas, increased levels of ROS and lipid peroxidation in liver, and decrease in CAT activity. BF treatment normalized all these parameters. BF did not normalize hyperglycemia, liver/body weight ratio, aspartate aminotransferase, protein carbonyl, NPSH levels, and δ-ALA-D activity. The raised oxidative stress seems to be a potential mechanism involved in liver damage in hyperglycemic conditions. Our results indicated that BF protective effect could be attributed to its antioxidant capacity, more than a hypoglycemic potential.