Oxidative Medicine and Cellular Longevity / 2016 / Article / Fig 2

Research Article

An Acetamide Derivative as a Camptothecin Sensitizer for Human Non-Small-Cell Lung Cancer Cells through Increased Oxidative Stress and JNK Activation

Figure 2

CPT and NPOA cotreatment induced apoptosis of H1299 cells. The cells were treated with 0.5 μM CPT alone, 10 μM NPOA alone, or their combination for 24 h and 48 h, respectively. (a) The cells were double-stained with Annexin V and PI and analyzed by flow cytometer-based detection assay. (b) The quantitative analysis of (a). Data are presented as means ± SD (). (c) The results of Western blot assay showed the changes of mitochondrial apoptotic Bax protein, cleaved caspase 9 and cleaved caspase 3, and full-length caspase 8. Abbreviations: C-caspase 9 indicates cleaved caspase 9 and C-caspase 3 indicates cleaved caspase 3. GAPDH as an internal control for equal loading.

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