An Acetamide Derivative as a Camptothecin Sensitizer for Human Non-Small-Cell Lung Cancer Cells through Increased Oxidative Stress and JNK Activation
CPT and NPOA cotreatment induced apoptosis of H1299 cells. The cells were treated with 0.5 μM CPT alone, 10 μM NPOA alone, or their combination for 24 h and 48 h, respectively. (a) The cells were double-stained with Annexin V and PI and analyzed by flow cytometer-based detection assay. (b) The quantitative analysis of (a). Data are presented as means ± SD (). (c) The results of Western blot assay showed the changes of mitochondrial apoptotic Bax protein, cleaved caspase 9 and cleaved caspase 3, and full-length caspase 8. Abbreviations: C-caspase 9 indicates cleaved caspase 9 and C-caspase 3 indicates cleaved caspase 3. GAPDH as an internal control for equal loading.
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