An Acetamide Derivative as a Camptothecin Sensitizer for Human Non-Small-Cell Lung Cancer Cells through Increased Oxidative Stress and JNK Activation
NPOA enhanced CPT-induced apoptosis through mitochondrial pathway. H1299 cells were treated with CPT alone and NPOA alone or their combination for 24 h, respectively. Afterward, H1299 cells were analyzed using flow cytometer- and fluorescence microscopy-based JC-1 assays, respectively. (a) The changes of mitochondrial membrane potential were determined by flow cytometer-based JC-1 staining assay. (b) The quantification analysis of (a). Data are presented as means ± SD (). (c) The green fluorescence of JC-1 indicates the decrease of mitochondrial membrane potential, a hallmark of apoptosis at the early stage. Magnification 200x.
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