Research Article
An Acetamide Derivative as a Camptothecin Sensitizer for Human Non-Small-Cell Lung Cancer Cells through Increased Oxidative Stress and JNK Activation
Figure 5
The ROS scavenger attenuated CPT and NPOA cotreatment-induced apoptosis of NSCLC cells. The H1299 cells were pretreated with 2 mM NAC for 3 h and then subject to cotreatment of CPT and NPOA for 24 h, as described in the Materials and Methods section. (a) The cells were double-stained with Annexin V and PI and analyzed by flow cytometer-based detection assay. (b) The quantitative analysis of (a). Data are presented as means ± SD ( > 0.05, < 0.001). (c) The results of photography showed the JC-1 green fluorescence image and indicated that NAC rescued the loss of mitochondrial membrane potential of H1299 cells after CPT and NPOA cotreatment. Magnification 200x.
(a) |
(b) |
(c) |