Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

Table 3

Scavenging effects of 1–5 on different in vitro assays.

Assay	Scavenging effects (IC50 µg/mL)
	1	2	3	4	5	Reference compounds
DPPH radical	39.39 ± 2.57	83.43 ± 5.11	12.49 ± 3.98	10.21 ± 4.23	15.45 ± 7.51	Ascorbic acid; 54.14 ± 2.87; Gallic acid; 3.76 ± 0.82
NO radical	48.69 ± 6.27	85.04 ± 4.83	30.29 ± 3.48	25.47 ± 4.70	31.52 ± 2.93	Ascorbic acid; 31.27 ± 5.21; Quercetin; 17.30 ± 1.98
	8.87 ± 1.05	18.86 ± 2.19	6.03 ± 0.39	5.76 ± 0.73	7.21 ± 3.22	Ascorbic acid; 5.11 ± 0.48
Hydroxyl radical	44.13 ± 4.28	62.34 ± 3.78	37.45 ± 2.58	33.17 ± 6.83	36.52 ± 5.40	Ascorbic acid; 30.29 ± 6.17
Hydrogen peroxide	91.81 ± 4.57	143.13 ± 7.55	80.73 ± 6.76	87.68 ± 9.43	101.03 ± 10.88	Ascorbic acid; 443.76 ± 7.66
	Scavenging effects (%)
Chelating activity; 50 µg/mL	76	43	89	84	85	Ascorbic acid; 91
Peroxidationof BSA						Ascorbic acid; 94
100 µg/mL	30	25	36	38	32
200 µg/mL	55	48	60	64	59
300 µg/mL	83	65	90	97	92
	TEAC (nM)
TEAC: ABTS	28.11 ± 2.48	49.51 ± 4.15	19.38 ± 3.29	16.23 ± 2.15	20.34 ± 2.17	Curcumin; 12.04 ± 4.19

The data represent the means ± SD of three determinations. IC₅₀: concentration required to inhibit 50% of the activity; DPPH: 2,2-diphenyl-1-picrylhydrazyl; : superoxide radical; TEAC: ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging capacity in Trolox equivalence (nanomoles); BSA: bovine serum albumin; Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.