Research Article

Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

Table 4

Effect of 1–5 on cellular oxidation stress reduction activity.

Group DCF fluorescence (%)

Blank0.7 ± 0.004
Control89.2 ± 3.32
1
 10 µg/mL79.4 ± 5.32
 50 µg/mL64.3 ± 1.83
 100 µg/mL44.6 ± 4.39
2
 10 µg/mL86.5 ± 6.17
 50 µg/mL68.4 ± 5.38
 100 µg/mL55.2 ± 7.35
3
 10 µg/mL72.3 ± 7.09
 50 µg/mL60.8 ± 4.21
 100 µg/mL39.6 ± 3.19
4
 10 µg/mL69.8 ± 3.26
 50 µg/ml52.1 ± 2.99
 100 µg/mL35.1 ± 2.67
5
 10 µg/mL73.4 ± 5.16
 50 µg/mL58.5 ± 6.43
 100 µg/mL38.3 ± 4.29
Ascorbic acid 25 μg/mL19.1 ± 1.46

Fluorescence (488 nm (excitation) and 535 nm (emission)) was finally quantified after 24 h incubation. Data is shown as mean ± SD of the experiments (3 replicates each).