Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice
Table 4
Effect of 1–5 on cellular oxidation stress reduction activity.
Group
DCF fluorescence (%)
Blank
0.7 ± 0.004
Control
89.2 ± 3.32
1
10 µg/mL
79.4 ± 5.32
50 µg/mL
64.3 ± 1.83
100 µg/mL
44.6 ± 4.39
2
10 µg/mL
86.5 ± 6.17
50 µg/mL
68.4 ± 5.38
100 µg/mL
55.2 ± 7.35
3
10 µg/mL
72.3 ± 7.09
50 µg/mL
60.8 ± 4.21
100 µg/mL
39.6 ± 3.19
4
10 µg/mL
69.8 ± 3.26
50 µg/ml
52.1 ± 2.99
100 µg/mL
35.1 ± 2.67
5
10 µg/mL
73.4 ± 5.16
50 µg/mL
58.5 ± 6.43
100 µg/mL
38.3 ± 4.29
Ascorbic acid 25 μg/mL
19.1 ± 1.46
Fluorescence (488 nm (excitation) and 535 nm (emission)) was finally quantified after 24 h incubation. Data is shown as mean ± SD of the experiments (3 replicates each).