Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes

Figure 3

Cholesterol overload induces cell morphology changes. Cell morphology determined by transmission electron microscopy of CW and HC cells under ethanol (100 mM) and acetaldehyde (200 μM) treatments. M: mitochondria; ER: endoplasmic reticulum; : mitochondria fission; black arrow: autophagosome. Bar 200 nm. Images are representative of at least three independent experiments. (g) Microsomal cholesterol determination. Each column represents mean ± SEM of at least four independent experiments. Differences were considered significant at versus CW cells.

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