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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 9370565, 10 pages
http://dx.doi.org/10.1155/2016/9370565
Research Article

Behavior of Oxidative Stress Markers in Alcoholic Liver Cirrhosis Patients

1HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico
2Departamento de Farmacología, Facultad de Medicina Mexicali, UABC, Mexicali, BC, Mexico
3Liver Clinic, Gastroenterology Service, Hospital General de México, Mexico City, Mexico
4Blood Bank Service, Hospital General de México, Mexico City, Mexico
5Liver Unit and Molecular Medicine, University Hospital, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico
6Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico

Received 12 August 2016; Revised 12 October 2016; Accepted 24 October 2016

Academic Editor: Sharad S. Singhal

Copyright © 2016 Marina Galicia-Moreno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Alcohol is the most socially accepted addictive substance worldwide, and its metabolism is related with oxidative stress generation. The aim of this work was to evaluate the role of oxidative stress in alcoholic liver cirrhosis (ALC). This study included 187 patients divided into two groups: ALC, classified according to Child-Pugh score, and a control group. We determined the levels of reduced and oxidized glutathione (GSH and GSSG) and the GSH/GSSG ratio by an enzymatic method in blood. Also, protein carbonyl and malondialdehyde (MDA) content were estimated in serum. MDA levels increased in proportion to the severity of damage, whereas the GSH and GSSG levels decreased and increased, respectively, at different stages of cirrhosis. There were no differences in the GSH/GSSG ratio and carbonylated protein content between groups. We also evaluated whether the active consumption of or abstinence from alcoholic beverages affected the behavior of these oxidative markers and only found differences in the MDA, GSH, and GSSG determination and the GSH/GSSG ratio. Our results suggest that alcoholic cirrhotic subjects have an increase in oxidative stress in the early stages of disease severity and that abstinence from alcohol consumption favors the major antioxidant endogen: GSH in patients with advanced disease severity.