The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 3

¹²⁵I seeds radiation induced autophagy in HCT116 cells. (a) HCT116 cells were exposed to the indicated dose of ¹²⁵I seeds radiation. LC3 punctuation was measured using immunofluorescence with polyclonal anti-LC3 antibody. DAPI was used to stain nuclei. (b) The number of LC3 punctuations per cell after exposure to indicated doses of ¹²⁵I seeds radiation was quantified. The values are the mean (±SD) of at least three different cells. (c) HCT116 cells were exposed to the gradually increased dose of ¹²⁵I seeds radiation. The ratio of LC3II/I was analyzed by Western analysis with anti-LC3 antibody. β-Actin was evaluated as a loading control.

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