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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 9780193, 5 pages
http://dx.doi.org/10.1155/2016/9780193
Research Article

A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops

1Faculty of Medicine, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, Brazil
2Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil
3Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil
4Laboratory of Cardiovascular Physiology, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil
5Department of Ophthalmology, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, Brazil

Received 3 June 2016; Accepted 21 July 2016

Academic Editor: Alessandra Ricelli

Copyright © 2016 Patrícia Ioschpe Gus et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Importance. Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature. Objective. This study sought to evaluate the total reactive antioxidant potential (TRAP) and concentration of reactive oxygen species (ROS) in samples of 50% AS eye drops and their correlations with the demographic characteristics and lifestyle habits of patients with ocular surface disease and healthy controls. Design. This was a case-control study with a 3-month follow-up period. Participants. 16 patients with severe dry eye disease of different etiologies and 17 healthy controls matched by age, gender, and race were included. Results. TRAP and ROS were detected at all evaluated times. There were no differences in the mean ROS () or TRAP () levels between cases and controls. No statistically significant differences in the concentrations of ROS or TRAPs were found at 0, 15, or 30 days ( for ROS = 0.087 and for TRAP = 0.93). Neither the demographic characteristics nor the lifestyle habits were correlated with the oxidative balance of the 50% AS eye drops. Conclusions and Relevance. Both fresh and frozen 50% AS eye drops present antioxidant capacities and ROS in an apparently stable balance. Moreover, patients with ocular surface disease and normal controls produce equivalent AS eye drops in terms of oxidative properties.