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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 9895176, 13 pages
Research Article

Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

1Postgraduate Program in Experimental Biology, DCBS, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, DF, Mexico
2Health Science Department, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, DF, Mexico
3Translational Research Unit, Medica Sur Clinic and Foundation, 14050 Mexico City, DF, Mexico
4Department of Cell Death and Proliferation, Instituto Investigaciones Biomédicas de Barcelona, CSIC, Barcelona and Liver Unit-Hospital Clinic-IDIBAPS and Centro de Investigación Biomédica en Red (CIBERehd), 08036 Barcelona, Spain
5Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), University of Salamanca, Centro de Investigación Biomédica en Red (CIBERehd), Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007 Salamanca, Spain
6Red “Fisiopatología de Enfermedades Hepáticas” PRODEP-SEP, 09340 Mexico City, DF, Mexico
7General Surgery Service 304, General Hospital of Mexico, UNAM School of Medicine, 06726 Mexico City, DF, Mexico
8Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

Received 2 May 2016; Accepted 13 July 2016

Academic Editor: Ravirajsinh Jadeja

Copyright © 2016 Natalia Nuño-Lámbarri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1. A) Experimental design for the animal studies. B) Liver gross inspection from animals fed with control diet supplemented with sodium cholate 0.5% (Cholate), or with the high cholesterol diet (HC) for 2 and 30 days. C) Liver/Body weight ratio, AST and ALT serum activities of animals under cholate (0.5%) alone diet or HC for 2 and 30 days. Each column represents mean ± SEM of three independent experiments. Differences were considered significant at * p ≤ 0.05 vs Chow animals.

Supplementary figure 2. Antibodies used in the study.

Supplementary figure 3. A high cholesterol diet induces hepatocyte free cholesterol and neutral lipids overload. Hepatocytes were isolated from animals fed with Standard control diet (Chow) or High cholesterol diet (HC). A-B) Free cholesterol determination by filipin, C-D) Neutral lipid determined by Oil Red O staining (ORO), Biochemistry determination of E) total cholesterol; and F) triglycerides (TG) content. Differences were considered significant at * p ≤ 0.01 vs Chow. Images are representative of at least three independent experiments. Original magnification 200X.

  1. Supplementary Material