Oxidative Phosphorylation System in Gastric Carcinomas and Gastritis

Figure 6

Localization of VacA in gastritis. (a) Triple immunofluorescence staining (complex I/VacA/DAPI) of case BGM-4 revealed strong HP colonization of the gastric lumen (10x magnification). (b) Triple immunofluorescence staining (porin/VacA/DAPI) of case BG-3 revealed selective staining of mitochondria-rich parietal cells. No differences in porin expression between the infected and noninfected parietal cells were detected. Parietal cells (white arrow) and mucous-secreting cells (yellow arrow) (63x magnification). (c) Triple immunofluorescence staining (TFAM/VacA/DAPI) of case BG-3 showed intracellular staining of VacA in parietal cells. (d) Z-stack of triple immunofluorescence staining (TFAM/VacA/DAPI) of case BG-1 revealed cytoplasmic localization of VacA (63x magnification). (e) Intraglandular VacA in case BG-1 (TFAM/VacA/DAPI); (20x magnification). (f) Triple immunofluorescence staining (Porin/VacA/DAPI) of case BG-3. (g) Figure f without the green channel to better visualize the intraglandular (white arrow) and intraparietal (yellow arrow) localization of VacA.

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