Rat Liver Enzyme Release Depends on Blood Flow-Bearing Physical Forces Acting in Endothelium Glycocalyx rather than on Liver Damage
Table 1
Intracellular distribution of enzyme activities determined in livers from sham-operated and PH animals.
LDH
ALT
AST
OCT
GDH
Cytosol (% of total activity)
Sham
75 ± 5
73 ± 6
44 ± 5
8 ± 1
0.6 ± 0.1
PH, 12 h
53 ± 7
58 ± 12
32 ± 7
3 ± 0.4
0.6 ± 0.1
PH, 24 h
51 ± 4
76 ± 10
45 ± 7
7 ± 1
1.0 ± 0.2
PH, 48 h
57 ± 8
72 ± 10
39 ± 5
6 ± 1
0.7 ± 0.2
PH, 72 h
62 ± 12
68 ± 11
35 ± 6
5 ± 1
0.4 ± 0.1
Mitochondria (% of total activity)
Sham
2.5 ± 0.4
3 ± 0.5
28 ± 4
71 ± 9
66 ± 6
PH, 12 h
18 ± 3
5 ± 0.7
46 ± 8
90 ± 4
78 ± 11
PH, 24 h
14 ± 2
3 ± 0.6
35 ± 5
64 ± 5
63 ± 4
PH, 48 h
9 ± 2
3 ± 0.6
36 ± 5
69 ± 6
69 ± 6
PH, 72 h
6 ± 0.8
2.5 ± 12
33 ± 4
72 ± 8
67 ± 9
Microsomes (% of total activity)
Sham
16 ± 1
8 ± 1
10 ± 2
1.5 ± 0.4
15 ± 2
PH, 12 h
20 ± 3
8 ± 1
8 ± 1
1 ± 0.1
6 ± 2
PH, 24 h
28 ± 3
8 ± 1
11 ± 2
3.4 ± 0.6
18 ± 2
PH, 48 h
22 ± 3
10 ± 2
11 ± 2
2.4 ± 0.5
15 ± 3
PH, 72 h
21 ± 3
9 ± 2
12 ± 3
1.3 ± 0.2
13 ± 2
Plasma membranes (% of total activity)
Sham
6 ± 1
10 ± 1
16 ± 3
19 ± 3
17 ± 3
PH, 12 h
9 ± 2
15 ± 2
10 ± 2
6 ± 1
14 ± 3
PH, 24 h
7 ± 1
9 ± 1
8 ± 2
25 ± 4
15 ± 2
PH, 48 h
9 ± 2
13 ± 2
12 ± 2
23 ± 3
19 ± 3
PH, 72 h
11 ± 2
15 ± 2
18 ± 3
21 ± 4
18 ± 2
Nuclei (% of total activity)
Sham
0.14 ± 0.02
6 ± 1
2.0 ± 0.3
0.1 ± 0.03
1.5 ± 0.2
PH, 12 h
0.07 ± 0.02
12 ± 2
3.3 ± 0.7
0.2 ± 0.04
1.1 ± 0.2
PH, 24 h
0.23 ± 0.04
4 ± 1
1.5 ± 0.3
0.8 ± 0.10
2.1 ± 0.2
PH, 48 h
0.18 ± 0.03
6 ± 1
1.8 ± 0.3
0.3 ± 0.06
1.8 ± 0.3
PH, 72 h
0.11 ± 0.02
7 ± 1
2.3 ± 0.3
0.2 ± 0.02
1.5 ± 0.2
Percentages for enzyme activities are the mean ± SE of seven individual observations per experimental point for sham-operated control of partial hepatectomized (PH) rats. Statistical significance: against the control group.
