Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently

Table 3

Apparent K_m (^appK_m) and maximal rate of respiration (V_max) values for ADP-dependent respiration calculated for HBC, HCC and their adjacent healthy tissue samples.

Tissues	^appKm, μM ± SEM	Vmax ± SEM
Human breast cancer tissue	114.8 ± 13.6	1.09 ± 0.04
Healthy adjacent breast control tissue	—	0.02 ± 0.01
Human colorectal cancer tissue	93.6 ± 7.7	2.41 ± 0.32
Healthy adjacent colon control tissue	256 ± 34	0.71 ± 0.23

Note: from [26] and [38]; V_max values are presented as nM O₂/min/mg dry tissue weight without proton leak rates. These K_m and V_max values for ADP were determined from corresponding titration curves by fitting experimental data to non-linear regression equation according to a Michaelis–Menten model. 35 patients used for analysis of HBC and 35 for HCC.