Diallyl Trisulfide Suppresses Oxidative Stress-Induced Activation of Hepatic Stellate Cells through Production of Hydrogen Sulfide

Figure 3

DATS stimulates apoptosis associated with H₂S in HSCs. (a) Hoechst fluorescence staining for morphology of apoptotic HSCs under a fluorescence microscope (200x magnification). (b, c) Flow cytometric analyses of apoptotic rates. Significance: versus DMSO + H₂O₂ and versus DMSO + H₂O₂. (d, e) Western blot analyses of apoptosis regulatory proteins.

(a)

(b)

(c)

(d)

(e)