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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 1502489, 14 pages
https://doi.org/10.1155/2017/1502489
Research Article

Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos

1Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, SP, Brazil
2Institute of Biomedical Sciences, Universidade de São Paulo, SP, Brazil
3Institute of Biosciences, Campus Botucatu, Department of Pharmacology, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil
4School of Sciences and Languages, Campus Assis, Department of Biological Sciences, Universidade Estadual Paulista (UNESP), Assis, SP, Brazil

Correspondence should be addressed to Marcella Pecora Milazzotto

Received 18 April 2017; Revised 4 August 2017; Accepted 21 August 2017; Published 25 October 2017

Academic Editor: Serafina Perrone

Copyright © 2017 Roberta Ferreira Leite et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O2 group and the number of cells was assessed, with the 5% O2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.