Figure 4: Effect of MFE on EC-induced GSH depletion in HepG2 cells. (a) Cells were pretreated with different concentrations of MFE (0.5 mg/mL, 1 mg/mL, and 2 mg/mL) for 24 h and then incubated with EC for another 24 h. After that, cells were collected and incubated with 50 μM of NDA at 37°C for 30 min then washed with PBS and evaluated by fluorescence microscope. (b) The quantitative data of panel (a) and results were expressed as mean NDA fluorescence intensity ± standard deviations. Different letters represent significant difference (). EC, ethyl carbamate; MFE, mulberry fruit extract; GSH, glutathione; NDA, naphthalene-2.