Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 1

Research Article

Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment

Figure 1

Effects of dicarbonyls and thrombin on the transendothelial electric resistance of the EA.hy926 cell monolayer. (a) Time course of dose-dependent TER changes produced by the indicated MDA concentrations and 100 nM thrombin (Thr); (b) time course of TER change produced by 250 μM MDA, GO, MGO, or glutaraldehyde (GA); (c) effects of MDA washout on the time course of TER decline produced by 200 μM MDA; (d) time course of TER change produced by 200 μM MDA in the presence of 0.5 mM NAC, lysine, or carnosine and in the presence of 2 mM lysine or carnosine. Control, cells treated with HBSS without dicarbonyls added. The arrow indicates the addition of either dicarbonyl (a–d) and thrombin (a) or MDA washout (c). TER is expressed as the normalized impedance. Data from three independent experiments were pooled. versus control, MGO, GO, or thrombin for the marked conditions at 20 hours (). $ versus both control and MDA at 10 hours (). # versus both control and MDA at 10 hours (). & versus MDA at 10 hours ().

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.