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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 1682393, 8 pages
Research Article

The Stimulated Glycolytic Pathway Is Able to Maintain ATP Levels and Kinetic Patterns of Bovine Epididymal Sperm Subjected to Mitochondrial Uncoupling

1Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil
2Department of Pharmacology, Faculty of Medicine, Autonomous University of Madrid, Madrid, Spain
3Department of Medical Science, Faculty of Medicine Ciudad Real, University of Castilla-La Mancha, Ciudad Real, Spain
4School of Veterinary Medicine and Animal Science, Avenue Orlando Marques de Paiva, No. 87, 05508-270 São Paulo, SP, Brazil

Correspondence should be addressed to Marcilio Nichi; rb.psu@ihcinm

Received 3 February 2017; Accepted 8 March 2017; Published 9 May 2017

Academic Editor: Moh H. Malek

Copyright © 2017 João D. A. Losano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Studies have reported the importance of mitochondria in sperm functionality. However, for some species, the glycolytic pathway appears to be as important as oxidative phosphorylation in ATP synthesis and sperm kinetics. These mechanisms have not been fully elucidated for bovine spermatozoa. Therefore, the aim of this study was to evaluate the role of mitochondria and the glycolytic pathway in ATP synthesis, sperm movement patterns, and oxidative homeostasis of epididymal spermatozoa in bovine specimens. We observed that mitochondrial uncoupling with protonophores significantly reduced ATP levels. However, these levels were reestablished after stimulation of the glycolytic pathway. We verified the same pattern of results for sperm kinetic variables and the production of reactive oxygen species (ROS). Thus, we suggest that, after its appropriate stimulation, the glycolytic pathway is capable of maintaining ATP levels, sperm kinetic patterns, and oxidative balance of bovine epididymal spermatozoa submitted to mitochondrial uncoupling.