Caffeic Acid Phenethyl Ester Reduces Ischemia-Induced Kidney Mitochondrial Injury in Rats

Figure 9

Ultrastructural changes to mitochondria. (a, b) Control. Mitochondria display normal morphology—shape is from round to elongate depending on the section, cristae are arranged in parallel rows at regular intervals, and the matrix is evenly dense. Note that normal morphology is characteristic to all mitochondria despite their irregular distribution in renal cells. (c, d) 20 min ischemia affected mitochondria: matrix is enlarged, uneven, and partly interrupted by empty patches; the outer and inner mitochondrial membranes are close and parallel. In 22 mg/kg CAPE group (c), cristae are parallel ranged; however, in CAPE 34 mg/kg group (d), irregular orientation of cristae can be seen. (e, f) 40 min ischemia affected mitochondria. (e) Cell with moderately preserved mitochondria in CAPE 22 mg/kg group: matrix is slightly enlarged with seldom empty patches, cristae are irregularly oriented, and the outer membrane is discontinuous. Note a mitochondrion with fully lost inner structure (). (f) Mitochondria in CAPE 34 mg/kg group possess noticeably swollen matrix and exclusively peripheral cristae, while the outer membrane is ruptured in many places. N: nucleus; RER: rough endoplasmic reticulum; Ex: extracellular space. Scale bar: 1 μm.

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