Research Article

HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages

Figure 3

Effects of HO-1 on TSG mediated anti-inflammatory effect in LPS-activated macrophages. RAW264.7 cells were pretreated with TSG (120 μM) for 6 h with or without 20 μM of ZnPP and then stimulated with LPS (1 μg/mL) for 12 h. (a-b) Relative mRNA expression of TNF-α and IL-6 was determined by Real-Time PCR. (c) Western blot analysis was performed to assess the expression of COX-2, IL-6, and TNF- in whole cell lysates. (d) The relative IL-6 and TNF- protein level was analyzed by densitometry. Data were expressed as the mean ± SEM of triplicate independent experiments (). , , and versus control group; # versus TSG treatment group; versus LPS + TSG group.
(a)
(b)
(c)
(d)