HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages

Figure 6

TSG restored mitochondrial function in LPS-treated RAW264.7 cells via an HO-1 dependent fashion. RAW264.7 cells were pretreated with TSG (120 μM) for 6 h with or without 20 μM of ZnPP and then stimulated with LPS (1 μg/mL) for 12 h. (a) After treatment, the mRNA expressions of PGC-1, NRF1, and TFAM were detected by Real-Time PCR. (b) Quantitative PCR was performed to evaluate the mtDNA content. Relative ratios of mtDNA and nDNA contents were analyzed. (c) The mitochondrial complex I activity was detected by following the oxidation of NADH with a specific detecting kit. (d) The cellule ATP content was analyzed with a luciferase assay kit. (e) The superoxide anion content in RAW264.7 cells was detected by flow-cytometry method after staining with MitoSOX. (f) The MitoSOX fluorescence was analyzed by using Image-Pro plus 6 software. Data were expressed as the mean ± SEM of triplicate independent experiments (). and versus control group; ^# versus TSG group; versus LPS + TSG group.

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