YiQiFuMai Powder Injection Protects against Ischemic Stroke via Inhibiting Neuronal Apoptosis and PKCδ/Drp1-Mediated Excessive Mitochondrial Fission

Figure 3

YQFM protects primary cortical neurons from H₂O₂-induced apoptosis. (a) Neurons were treated with different concentrations of YQFM (25–800 μg/ml) for 24 h. Cell viability was measured using MTT assay. (b) Neurons were pretreated with YQFM (25–800 μg/ml) or NAC (500 μM) for 6 h before addition of H₂O₂ (100 μM) for 12 h. Cell viability was measured using MTT assay. Neurons were pretreated with YQFM (100, 200, and 400 μg/ml) or NAC (500 μM) for 6 h before exposure of H₂O₂ (100 μM) for 12 h. (c) Caspase-3 activity was assayed using the caspase-3 activity assay kit. (d) Protein level of cleaved caspase-3 was detected by Western blot. (e) Flow cytometric analysis of Annexin V-FITC/PI stained neurons and quantification as the percentage of apoptotic cells. Results were expressed as mean ± SD from three independent experiments. versus control, versus control, versus H₂O₂, versus H₂O₂.

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