Oxidative Stress Delays Prometaphase/Metaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint

Figure 3

H3S10P detection in mouse zygotes. H3S10P (green) expression during S phase, prometaphase, and anaphase in control zygotes at 18.0, 19.0, and 20.5 hpi, respectively. The late G2 phase, metaphase, and telophase visualizations were obtained from treated zygotes at 21.0, 22.0, and 23.5 hpi, respectively. Nuclei were stained with DAPI (blue).