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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 2190897, 11 pages
Research Article

Methane Suppresses Microglial Activation Related to Oxidative, Inflammatory, and Apoptotic Injury during Spinal Cord Injury in Rats

1Department of Orthopaedics, Changzheng Hospital Affiliated to the Second Military Medical University, Shanghai 200003, China
2Department of Anesthesiology, Changzheng Hospital Affiliated to the Second Military Medical University, Shanghai 200003, China
3Institute of Biomedical Engineering, Second Military Medical University, Shanghai 200433, China

Correspondence should be addressed to Xiaojian Ye

Received 5 February 2017; Revised 18 March 2017; Accepted 29 March 2017; Published 27 June 2017

Academic Editor: Wei Chen

Copyright © 2017 WeiHeng Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. We investigated the hypothesis that methane-rich saline (MS) can be used to repair spinal cord injury (SCI) in a rat model through suppressing microglial activation related to oxidative, inflammatory, and apoptotic injury. Methods. MS was injected intraperitoneally in rats after SCI. Hematoxylin-eosin (HE) staining, oxidative stress, inflammatory parameters, and cell apoptosis were detected 72 h after SCI to determine the optimal dose. Then, we investigated the protective mechanisms and the long-term effects of MS on SCI. HE and microglial activation were observed. Neurological function was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale. Results. MS can significantly decrease infarct area and inhibit oxidative stress, inflammation, and cell apoptosis 72 h following SCI. The MS protective effect at a dose of 20 ml/kg was better. Moreover, MS can significantly suppress microglial activation related to oxidative and inflammatory injury after SCI and improve hind limb neurological function. Conclusion. MS could repair SCI and reduce the release of oxidative stress, inflammatory cytokines, and cell apoptosis produced by activated microglia. MS provides a novel and promising strategy for the treatment of SCI.