Oxidative Medicine and Cellular Longevity

Research Article

Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach

Table 1

List of differentially expressed proteins identified by MS/MS in CTR and P1 samples.


Spot number	Swiss-Prot accession number	Protein name	Gene name	Mascot score		Sequence coverage MS	Sequence coverage MS/MS	Peptides	Fold change P1/CTR	value	Instrument

Spot 1	Q9Y4L1	Hypoxia-upregulated protein (HYOU1)	HYOU1	181	106	25%	2%	K.LCQGLFFR.V K.QADNPHVALYQAR.F	2.2		MALDI-TOF/TOF
Spot 2	Q06830	Peroxiredoxin-1 (PRDX1)	PRDX1	/	47	/	29%	R.TIAQDYGVLK.A K.ATAVMPDGQFK.D + oxidation (M) R.LVQAFQFTDK.H R.QITVNDLPVGR.S	1.6		ESI-Trap
Spot 3	P09936	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1)	UCHL1	86	220	49%	19%	R.LGVAGQWR.F R.MPFPVNHGASSEDTLLK.D + oxidation (M) K.NEAIQAAHDAVAQEGQCR.V	3.8		MALDI-TOF/TOF
Spot 4	P63244	Guanine nucleotide-binding protein subunit beta-2-like 1 (GBLP)	GNB2L1	97	84	38%	6%	R.VWQVTIGTR.-R.DETNYGIPQR.A	2.3		MALDI-TOF/TOF
Spot 5	Q8WUM4	Programmed cell death 6 interacting protein (PDC6I)	PDCD6IP	175	167	29%	4%	R.TPSNELYKPLR.A R.YYDQICSSIEPK.F K.MVPVSVQQSLAAYNQR.K	1.6		MALDI-TOF/TOF
Spot 6	P15311	Ezrin (EZRI)	EZR	111	134	25%	4%	K.IGFPWSEIR.N R.IQVWHAEHR.G K.KAPDFVFYAPR.L	−5.5		MALDI-TOF/TOF
Spot 7	P17987	T-complex protein 1 subunit alpha (TCPA)	TCP1	96	214	22%	6%	K.YFVEAGAMAVR.R K.IHPTSVISGYR.L R.YINENLIVNTDELGR.D	−1.6		MALDI-TOF/TOF
Spot 8	P34897	Serine hydroxymethyltransferase mitochondrial (GLYM)	SHMT2	61	142	27%	9%	R.LIIAGTSAYAR.L K.TGLIDYNQLALTAR.L R.GYSLVSGGTDNHLVLVDLRPK.G	1.8		MALDI-TOF/TOF
Spot 9	Q16658	Fascin (FSCN1)	FSCN1	150	171	42%	6%	R.FLIVAHDDGR.W K.LINRPIIVFR.G K.NASCYFDIEWR.D	−1.7		MALDI-TOF/TOF
Spot 10	P49368	T-complex protein 1 subunit gamma (TCPG)	CCT3	87	145	12%	6%	R.NLQDAMQVCR.N R.TLIQNCGASTIR.L K.AMTGVEQWPYR.A + oxidation (M)	−1.8		MALDI-TOF/TOF
Spot 11	P07741	Adenine phosphoribosyltransferase (APT)	APRT	60	147	28%	21%	R.IDYIAGLDSR.G R.SFPDFPTPGVVFR.D K.AELEIQKDALEPGQR.V	1.6		MALDI-TOF/TOF
Spot 12	P07195	L-lactate dehydrogenase B chain (LDH-B)	LDHB	125	127	33%	11%	K.IVVVTAGVR.G R.VIGSGCNLDSAR.F K.GEMMDLQHGSLFLQTPK.I + oxidation (M)	1.9		MALDI-TOF/TOF
Spot 13	P35998	26S proteasome regulatory subunit 7 (PRS7)	PSMC2	61	67	20%	9%	R.KIEFSLPDLEGR.T K.QTLQSEQPLVAR.C K.ACLIFFDEIDAIGGAR.F	1.8		MALDI-TOF/TOF
Spot 14	P07355	Annexin A2 (ANXA2)	ANXA2	67	82	21%	7%	K.WISIMTER.S + oxidation (M) K.AYTNFDAER.D R.QDIAFAYQR.R	2		MALDI-TOF/TOF
Spot 15	P00338	L-lactate dehydrogenase A chain (LDHA)	LDHA	96	83	26%	7%	K.LVIITAGAR.Q K.DQLIYNLLKEEQTPQNK.I	2		MALDI-TOF/TOF

Spot numbers match those reported in the representative 2-DE images shown in Figure 1. Accession number in Swiss-Prot/UniprotKB (http://www.uniprot.org/). Fold change (P1 versus CTR cells) was calculated dividing the average of %V P1 cells by the average of %V CTR cells of three independent experiments. t-test was performed by GraphPad v4.0 software to determine if the relative change was statistically significant (); ; ; .