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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 2353729, 12 pages
https://doi.org/10.1155/2017/2353729
Research Article

Increased Oxidative Damage of RNA in Early-Stage Nephropathy in db/db Mice

1The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
2Department of Laboratory Medicine, Gansu Provincial Hospital, Lanzhou, Gansu 730000, China
3Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Dongdan, Beijing 100730, China
4Department of Clinical Pharmacy, The People’s Hospital of Lishui, Lishui 323000, China
5Department of Clinical Pharmacy, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo 315000, China
6Department of Nephrology, Beijing Hospital, Ministry of Health, Beijing 100730, China
7Department of Laboratory Medicine, Beijing Hospital of the Ministry of Health, Beijing 100730, China
8Department of Pharmacology, Wenzhou Medical University, University Town, Wenzhou, Zhejiang 325035, China

Correspondence should be addressed to Jian-Ping Cai; moc.anis.piv@16pjiac

Received 15 May 2017; Revised 3 September 2017; Accepted 12 September 2017; Published 19 October 2017

Academic Editor: Aiqing Li

Copyright © 2017 Wan-Xia Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To evaluate RNA oxidation in the early stage of diabetic nephropathy, we applied an accurate method based on isotope dilution high-performance liquid chromatography-triple quadruple mass spectrometry to analyze the oxidatively generated guanine nucleosides in renal tissue and urine from db/db mice of different ages. We further investigated the relationship between these oxidative stress markers, microalbumin excretion, and histological changes. We found that the levels of 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) were increased in the urine and renal tissue of db/db mice and db/db mice with early symptoms of diabetic nephropathy suffered from more extensive oxidative damage than lean littermate control db/m mice. Importantly, in contrast to the findings in db/m mice, the 8-oxoGuo levels in the urine and renal tissue of db/db mice were higher than those of 8-oxodGuo at four weeks. These results indicate that RNA oxidation is more apparent than DNA oxidation in the early stage of diabetic nephropathy. RNA oxidation may provide new insight into the pathogenesis of diabetic nephropathy, and urinary 8-oxoGuo may represent a novel, noninvasive, and easily detected biomarker of diabetic kidney diseases if further study could clarify its source and confirm these results in a large population study.