Research Article
Increased Oxidative Damage of RNA in Early-Stage Nephropathy in db/db Mice
Figure 2
Histological and ultra-structural characteristics in diabetic db/db mice. db/db represents BKS.Cg-Dock7m +/+ Leprdb/JNju mice, and db/m represents their lean littermates. Samples from 4-, 8-, 12-, and 16-week-old mice were examined, and the results are shown as 4 w, 8 w, 12 w, and 16 w, respectively. (a) Periodic acid-Schiff stain (original magnification, ×400) indicated a normal appearance in the outer cortex of control db/m mice and an increase in the mesangial matrix and glomerular hypertrophy in diabetic db/db mice. Scale bar: 25 μm. (b) An electron-microscopic image (original magnification, 8000x) shows a normal appearance of the glomerulus in db/m mice and podocyte effacement or loss and mesangial extracellular matrix expansion and irregular glomerular basement membrane (GBM) thickness (red arrow) in db/db mice. Scale bar: 2 μm. (c) GBM thickness (nanometers). (d) Podocyte foot process width (FPw) (nanometer). The GBM and FPw are shown as the means ± SD. (white-colored bars) db/m. (black-colored bars) db/db, versus respective control, # compared with the respective controls.
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