The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

Figure 4

Protective effect of BBR on H₂O₂-induced intracellular ROS production in human diploid fibroblasts. 2BS cells were pretreated with or without 12 μmol/L BBR prior to a 2 hr exposure of 200 μmol/L H₂O₂, or treated with BBR alone as the Materials and Methods mentioned. (a) The diagram of fluorescence DCF detection with different treatments from flow cytometry analysis. (b) Histogram of the mean fluorescence intensity of DCF, reflecting the percentages of ROS generation in different treatment group. . The results are representative of three separate experiments.

(a)

(b)