An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Figure 7

Effect of EDA in cellular morphology and SA-β-Gal of SIPSF cells. (a) SA-β-Gal was measured as nmol of fluorescein per number of cells in control (C, unexposed HFF cells), control 200 μM H₂O₂-exposed cells (C + H₂O₂), and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA measured at 24 h and 48 h after H₂O₂ exposition. (b) Cellular morphology in control cells (C13, cells from 13 PD; C45, cells from 45 PD; and C + H₂O₂) and H₂O₂-exposed cells treated with 0.3, 0.5, and 1 mg/mL EDA previous H₂O₂ exposure and 24 h after H₂O₂ recovery (lower line). The time of EDA treatment was 24 h before H₂O₂ exposition (PRE), just after H₂O₂ exposition (POST), and after and before H₂O₂ exposition (PRE-POST). Each bar represents the mean ± S.E. of three replicates from three independent experiments, and samples that do not have a common letter are significantly different in each condition (PRE, POST, and PRE-POST) by Duncan’s test at .

(a)

(b)