MicroRNA-93 Regulates Hypoxia-Induced Autophagy by Targeting ULK1

Figure 3

The knockdown of ULK1 by siRNA suppressed hypoxia-induced autophagy. (a, b) MEFs and CHO cells were transfected with three pairs of siRNAs to ULK for 24 h, respectively, si-ULK1 (488), si-ULK1 (2457), and si-ULK1 (3310), setting NC as a control. Cell lysates were prepared and subjected to Western blot analysis by using anti-ULK1 and anti-Gapdh. The densitometric ratios from the samples were quantified by using ImageJ. Data were from three independent experiments. Representative data are shown. (c) Si-ULK1 (488) was transfected into MEFs for 12 h, followed by hypoxic treatment or normoxic treatment for another 24 h. Cell lysates were analyzed by Western blot using anti-ULK1, anti-P62, anti-LC3, and anti-Gapdh. Densitometric ratios of the samples were quantified by using ImageJ. (d) MEFs were treated as the same as (c). Cells were fixed and immunostained by anti-ULK1 (green) and anti-P62 (red) antibodies. Scale bar, 20 μm.

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