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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 2734976, 16 pages
Research Article

Cytotoxicity, Oxidative Stress, Cell Cycle Arrest, and Mitochondrial Apoptosis after Combined Treatment of Hepatocarcinoma Cells with Maleic Anhydride Derivatives and Quercetin

1Departamento de Bioquímica y Sección de Graduados, Escuela Superior de Medicina del IPN, Ciudad de México, Mexico
2CONACYT, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez, OAX, Mexico
3Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, Mexico

Correspondence should be addressed to José Guadalupe Trujillo-Ferrara; xm.npi@ollijurtj and Verónica Rocío Vásquez-Garzón; xm.tycanoc@agzeuqsavrv

Received 22 February 2017; Revised 12 June 2017; Accepted 18 July 2017; Published 10 October 2017

Academic Editor: Christian Widmann

Copyright © 2017 Gabriela Carrasco-Torres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary 1. Effect of individual administration of Q, C1 and C2 on cytoskeletal actin and nuclear morphology in human liver cancer cells. A ), B ) HuH7 cells and C), D) HepG2 cells at 24 hours and 48 hours post-treatment. Nuclear staining of Hoechst is shown in cyan and staining of the action F by phalloidin is shown in red at a magnification of 40X. Quantification of pycnotic nuclei by Hoechst staining in cells. E), G) HepG2 cells and F), H) HuH7 cells at 24 hours and 48 hours post-treatment. All the data presented have a mean ± SEM of 3 experiments; the evaluations are performed using Tukey՚s one-way ANOVA to obtain significant differences (* P <0.05, ** P <0.01, *** P <0.001) with normalization based on the vehicle group treated with DMSO. Supplementary 2. Wound closure assay. Effect on cell migration at 24 hours post-treatment in liver cancer cells A ) HuH7 and B ) HepG2; treatment with C1+Q and C2+Q resulted in an average inhibition of 43.45% with respect to the vehicle group. Normal control, NC; vehicle, DMSO; quercetin, Q; 3′5-dimaleamylbenzoic acid, C1; 3′5-Dimaleimylbenzoic acid, C2. Quantification performed with ImageJ.

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