Oleate Prevents Palmitate-Induced Atrophy via Modulation of Mitochondrial ROS Production in Skeletal Myotubes

Figure 2

The effects of palmitic and oleic acid on morphological features of myonuclei. Differentiated C2C12 myotubes were treated with 0.1, 0.3, 0.5, and 1 mM palmitate or oleate for 36 h. (a) Myonuclei were stained with DAPI and were visualized by florescence microscope (magnification = ×64). (b) Total myonucleus number, (b) fragmented myonuclei, and (c) fragmented myonuclei (%) were quantified. Bar graph represented means ± SE. Scale bar = 20 μm. The data were analyzed using two-way ANOVA followed by Tukey’s post hoc test. versus NT. NT: nontreatment.

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